Former captain Lewis Ludlow will play his 250th game for Gloucester after being named in the back row for the visit of Harlequins on Saturday.
The openside, who returns from a hand injury, is not the only player to reach a milestone at Kingsholm, as winger Ollie Thorley is set for his 150th appearance for the Cherry & Whites.
Ludlow will be joined in the back row by PREM debutant, Deian Gwynne, and Will Trenholm.
Matias Alemanno and Freddie Thomas, back from international duty, pack down in the second row, with Ciaran Knight, Seb Blake and Kirill Gotovtsev in front of them.
In the backs, captain Tomos Williams combines with Ross Byrne once more, with Seb Atkinson also returning from injury to partner Will Joseph in midfield.
Thorley is joined by a second PREM debutant in Rob Russell and Ben Redshaw in the back three.
On the bench, Val Rapava Ruskin is back in a Gloucester matchday squad for the first time this season.
Meanwhile, the club have confirmed that scrum-half Caolan Englefield won’t play again in 2025. Englefield suffered a shoulder injury whilst playing for England A against Spain.
Gloucester v Harlequins:
15 Ben Redshaw
14 Rob Russell*
13 Will Joseph
12 Seb Atkinson
11 Ollie Thorley
10 Ross Byrne
9 Tomos Williams (C)
1 Ciaran Knight
2 Seb Blake
3 Kirill Gotovtsev
4 Freddie Thomas
5 Matias Alemanno
6 Deian Gwynne*
7 Lewis Ludlow
8 Will Trenholm
16 Kealan Freeman-Price*
17 Val Rapava Ruskin
18 Afo Fasogbon
19 Cam Jordan
20 Josh Basham
21 Mikey Austin
22 Charlie Atkinson
23 Jack Cotgreave
*Denotes Gallagher PREM debut.
